Giraffe Pictures, Images and Photos Sacrifices at no limits.

Monday, 30 March 2009.
(:

I'm halfway done with presentation, I'm upright happy now. (:
No more pathetic agonies, I sound so...( you know what).
Ahhh whatever, no point grieving over such stupid things. (:

Okay, now it's YGSPPPP !
There's YGSP today, of course I'm happy!
Okay firstly everything went wrong because the bus driver went around the campus TWICE.
You may think it's nothing much but.. I can tell you that Singapore Polytechnic is ten times our schoollllllllllllllll.
It's super big, for your information, and it's isn't good finding yourself shaking like crazy in the bua around the campus TWO TIMES.
That is soooo .. yea you know ... the burning sensation in your stomach, like you're going to throw up anytime.
Reached the labortories, there's like 3 professors 0.0
Okay nevermind, back to the topic.

Okay the workshop is super fun in all.
At the start it's totally... (zzzzzzzzz)
But after a while it was practical which was FUNNNN !
& you'll never believe what is used to identify DNA.
Yes, it's ENZYMES.
Which is like totally (inserts word here).
Okay nevermind back to the topic.

Firstly we are supposed to add 10uL enzymes into a 10uL DNA (5 samples).
For your information, 1uL =1/100 ML, which is like ..., you can imagine the apparatus we use is totally NEVER SEEN in school and super high-tech.
&& it's super hygienic too, aprons, gloves( dental gloves, smelly) were used although it's just a small experiment.
Next, placed in water bath and went for breaks, cakes and tapioca cakes, as we need to wait for the DNA to bath for 30 minutes.
Went back to lab, saw our DNA, added another 4uL of dye inside.
Evelyn , Charlene and I was like trembling because it was sooo difficult to stick the apparatus inside the super-small test tube.
For your information, it's like 40 times smaller than our school test-tubes.
So you can imagine, the apparatus is sooo big and you're supposed to stick the super-big microplettes( the appratus used to suck uL amounts of stuffs) into the super-small test tube.
Okay it's really super difficult.

Then finished, sucked the solution up into the microplettes again, then stick it into the gel.
This was even moreeeeee ...
Then hole of the gel was SOOOOOOOOOOOOOO small, the small was like 10000( no joking okay) times smaller than our shcool test-tubes and you can imagine was scared we were to try placing solution inside.
& we ended up poking wrongly two holes.
Oppps ;X
So we called the professor to place it for us.
& she placed it in like oh-so-easily-and-perfectly, and we were like having those 0.0 expression beside her thinking : "How did she do it? "
Okay, so we placed everything in, and wait to to spread.
While spreading, second part of theory, lecture.
The language used was real deep, but, managed to grasp some of the things she taught.
& I really find this DNA characteristics interesting :
3'... TCATGCAGCAT...5'
5'... AGTACGTCGTA... 3'

If you're smart enough you should figure out the pattern.
It's in our DNA, hoho !
This is called the Watson-crick basepairs.
Woohoo!
No paper around me( except homework) okay! :}
Smart girl ;XXX !
Okay nevermind, have to go now.
I love DNA :D
I love YGSP. :D

Posted by P.Yuhuiz. on 7:31 pm

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